Susceptibility of Ephestia cautella ( Walk.) to entomopathogenic fungus Verticillium lecanii (Zimm.) Preparation

Authors

  • Fadhel Dakhel Soltan, Assist. Lecturer مديرية تربية واسط

DOI:

https://doi.org/10.31185/eduj.Vol1.Iss29.164

Abstract

       The study conducted  in the laboratory of  Biology department – College of Science – University of wasit, aimed to evaluate the  efficacy of spores suspension concentrations of Verticillium lecanii (Zimm.) against  (eggs , larvae , pupae and adults) of moth Ephestia cautella (Walk.) by direct application to insect at   ( 25 ± 1 °C and 65 ± 5% R.h. and photoperiod 14 L:10 D h(.The results showed that all fungal suspension concentrations (103,105,107) spore/ml affected on different stages of insect after ( 2 ,5 , 10) day  of the treatment compared with control treatment.

The laboratory results revealed that the all concentrations of spores suspension of   V. lecanii affect the mortality of eggs of moth after 2 day of the treatment and the percentage of mortality were 100%, and shown that the percentage mortality of larval stages and pupae were  100%  when they were treated for 5 day at107spore/ml followed by the another treatments in concentrations of 105,103 spore/ml after 10 day of the treatment were  100%.

On the other hand, bioassays conducted to evaluate the effect of direct exposure of adults to the concentrations(103,105,107) spore/ml and it revealed that all adults ( males and females) were killed after 10 day of the treatment and the highest percentage of mortality in males and females was100% and the LC50 values of males  were 6.2 x104 and females 6.5x105 spore / ml.

 The results of this study were revealed the success and effectiveness of using the Verticillium lecanii fungus suspension at (103,105,107) spore/ml as a pathogen in the control of moth Ephestia cautella laboratory.

 

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Published

2018-01-16

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Articles